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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague
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This website store cookies which are necessary for the proper function of this website (strictly necessary) in your browser. With your consent, the website will also use and store in your browser other cookies for the purpose of anonymous analysis of website traffic (analytical) and for the personalization of advertising (marketing). You can withdraw or modify your consent at any time by opening the "Cookies settings" menu at the bottom of the website. You provide your consent to UCT Prague for the domain vscht.cz including 3rd level domains. Further information can be found on the Privacy Policy and Cookie Policy pages. [cookie-policy-necessary] => Necessary [cookie-policy-necessary-text] => These cookies are necessary for the website to function properly and therefore cannot be disabled. [cookie-policy-analytics] => Analytical [cookie-policy-analytics-text] => We use web analytics cookies to anonymously analyse website visits and usage. [cookie-policy-functional] => Functional [cookie-policy-functional-text] => These cookies allow the website to remember your language and the choices you make. We encourage you to confirm that you are more comfortable using the site. [cookie-policy-button-accept-all] => Accept all [cookie-choose-handler] => Custom settings [cookie-choose-handler-send] => Save custom settings [cookie-reject-all-handler] => Accept only the necessary [cookie-policy-button-customize] => Cookies settings [cookie-policy-marketing] => Marketing [cookie-policy-marketing-text] => These cookies are used to evaluate advertising campaigns and personalize ads. [kalendar_nadpis] => Calendar [cely_kalendar] => All events [stahnout] => ) [poduzel] => stdClass Object ( [50384] => stdClass Object ( [obsah] => [poduzel] => stdClass Object ( [50385] => stdClass Object ( [obsah] => [iduzel] => 50385 [canonical_url] => _clone_ [skupina_www] => Array ( ) [url] => [sablona] => stdClass Object ( [class] => [html] => [css] => [js] => [autonomni] => ) ) [50388] => stdClass Object ( [obsah] => [iduzel] => 50388 [canonical_url] => _clone_ [skupina_www] => Array ( ) [url] => [sablona] => stdClass Object ( [class] => [html] => [css] => [js] => [autonomni] => ) ) [50389] => stdClass Object ( [obsah] => [iduzel] => 50389 [canonical_url] => _clone_ [skupina_www] => Array ( ) [url] => [sablona] => stdClass Object ( [class] => [html] => [css] => [js] => [autonomni] => ) ) ) [iduzel] => 50384 [canonical_url] => _clone_ [skupina_www] => Array ( ) [url] => [sablona] => stdClass Object ( [class] => [html] => [css] => [js] => [autonomni] => ) ) [58015] => stdClass Object ( [obsah] => [poduzel] => stdClass Object ( [58016] => stdClass Object ( [nazev] => [seo_title] => PhD studies at UCT Prague [seo_desc] => [autor] => [autor_email] => [obsah] => [urlnadstranka] => [ogobrazek] => [pozadi] => [iduzel] => 58016 [canonical_url] => [skupina_www] => Array ( ) [url] => /home [sablona] => stdClass Object ( [class] => boxy [html] => [css] => [js] => $(function() { setInterval(function () { $('*[data-countdown]').each(function() { CountDownIt('#'+$(this).attr("id")); }); },1000); setInterval(function () { $('.homebox_slider:not(.stop)').each(function () { slide($(this),true); }); },5000); }); function CountDownIt(selector) { var el=$(selector);foo = new Date; var unixtime = el.attr('data-countdown')*1-parseInt(foo.getTime() / 1000); if(unixtime<0) unixtime=0; var dnu = 1*parseInt(unixtime / (3600*24)); unixtime=unixtime-(dnu*(3600*24)); var hodin = 1*parseInt(unixtime / (3600)); unixtime=unixtime-(hodin*(3600)); var minut = 1*parseInt(unixtime / (60)); unixtime=unixtime-(minut*(60)); if(unixtime<10) {unixtime='0'+unixtime;} if(dnu<10) {unixtime='0'+dnu;} if(hodin<10) {unixtime='0'+hodin;} if(minut<10) {unixtime='0'+minut;} el.html(dnu+':'+hodin+':'+minut+':'+unixtime); } function slide(el,vlevo) { if(el.length<1) return false; var leva=el.find('.content').position().left; var sirka=el.width(); var pocet=el.find('.content .homebox').length-1; var cislo=leva/sirka*-1; if(vlevo) { if(cislo+1>pocet) cislo=0; else cislo++; } else { if(cislo==0) cislo=pocet-1; else cislo--; } el.find('.content').animate({'left':-1*cislo*sirka}); el.find('.slider_puntiky a').removeClass('selected'); el.find('.slider_puntiky a.puntik'+cislo).addClass('selected'); return false; } function slideTo(el,cislo) { if(el.length<1) return false; var sirka=el.width(); var pocet=el.find('.content .homebox').length-1; if(cislo<0 || cislo>pocet) return false; el.find('.content').animate({'left':-1*cislo*sirka}); el.find('.slider_puntiky a').removeClass('selected'); el.find('.slider_puntiky a.puntik'+cislo).addClass('selected'); return false; } [autonomni] => 1 ) ) [103595] => stdClass Object ( [nazev] => [seo_title] => PhD orientation day [seo_desc] => [autor] => [autor_email] => [obsah] =>

 ◳ PHD_Orientation_Day_TV_EN (png) → (originál)

Come and learn what life as a PhD candidate is like.
Meet your fellow PhD candidates and get to know the people from various support offices who will be there to help you throughout your doctoral journey.

Get oriented in the new structure of doctoral studies at UCT Prague!

  • What are your obligations during the PhD?
  • What is expected from you and how can you prepare?
  • What are the financial opportunities?
  • What do projects, mentoring, and soft skills courses involve?
  • What other opportunities does UCT Prague offer?

You’ll be introduced to all available support departments and the offices that organize support activities and courses:


PROGRAMME

9:00 – 9:10     Welcome speech – Vice-Rector for Doctoral Studies, Prof. Michaela Rumlová *
9:10 – 9:30     Introducing the PhD Office – Mgr. Mili Losmanová, PhD Support
(incl. a short introduction of Faculty Offices) *
9:30 – 10:30     The Electronic PhD Guide TeamIng. Petra Špringer Šimonová *

* For this part of the program (9 - 10:30), come with your supervisor

BREAK – 15 min

10:45 – 11:00     University Ombudsperson – ThDr. Klára Muzikářová, Ph.D.
11:00 – 11:20     International Relations Office – Bc. Lenka Polanská
11:20 – 11:40      HR Department – Ing. Simona Pavelková

11:40 – 12:20     Open discussion with current doctoral candidates

LUNCH BREAK – 1 hour

13:30 – 15:00     WorkshopJan Froněk, Academic Coach:
“Roadmap to PhD”

BREAK – 15 min

15:15 – 15:30     Welcome Office – Ing. Anna Mittnerová and Ing. Jitka Tomanová 
15:30 – 15:50     Counselling and Career Centre – TBA
15:50 – 16:10     National Library of Technology – Ing. Naděžda Firsová, Ph.D.

16:10 – 16:30     Project Centre – Ing. Hana Štěpánková
16:30 – 16:50     Research and Technology Transfer Office – Ing. Kateřina Kovaříčková
16:50 – 17:10     Data Stewardship Team – Ing. Martin Schatz, Ph.D. and Data Stewards


Moderated by: PhD Support – Mili Losmanová, Office for Doctoral Studies
Representatives from each Faculty Office will also be present.


You will receive:

  • Adaptation plan
  • “Survival Guide: 15 Tips for Navigating Your PhD” leaflet
  • A timeline of your PhD journey – clearly defined responsibilities and the ideal timing for completing them
  • A list of all transferable skills courses available to doctoral candidates at UCT Prague

Hosted by the PhD Office
Start your PhD with a clear overview and strong support!


 

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Preparation of IDCAC 2025 is under way. Details coming soon.

--------------------------------------------------------------

 ◳ banner_var2 (png) → (originál)

We are pleased to welcome you on the website of The First International Doctoral Conference on Advances in Chemistry , a conference for PhD candidates organised (mainly) by PhD candidates.
The conference took place on November 9, 2024 . The in-person part took place at UCT Prague. Especially for foreign participants, we were happy to offer the possibility to present their contribution on-line  in a hybrid session.

 ◳ obrázek-20241109-123815-02de1ea5 (png) → (šířka 450px)

Sections (Chairpersons)

  • Organic & Inorganic Chemistry                                             (Pavel Kraina)
  • Biochemistry                                                                                (Anna Amirianová)
  • Environmental Chemistry                                                      (Annelie Kadlecová)
  • Analytical, Physical and Computational Chemistry   (Jan Vališ & Eva Pospíšilová)
  • Chemical Engineering and Technology                            (Jakub Staś)

Venue

The in-person part of the conference will be held in the Dejvice Campus, particularly in the buildings of the University of Chemistry and Technology, Prague (Technická 5, Prague 6, Czech Republic).
If you are further from our venue, we are happy to offer the possibility to present your contribution on-line in a hybrid session. Participants who enrolled in the virtual form will receive a link to join us in due time.

Registration

The registration, which was opend up to Oct 15, 2024, is now closed.

Abstract submision

Abstract were to be submitted by Oct 15, 2024 and the limit was 2500 symbols (including spaces;  names of authors and their respecitive affiliations are excluded from character count limit) and the abstract should not contain any graphics. The abstract will be published in a conference proceedings and an ISBN will be attached to it.

Fees

We are pleased to inform you, that thanks to our parntners, the conference is free of cherge and in-person participants will be able to enjoy complimentary coffee breaks and lunch at UCT Prague. So don't forget to let us know about your dietary preferences and restrictions in the registration.

Programme

The registration will be held between 9:30 and 9:45 in the building A of UCT Prague (Technická 5, Prague), however participants are free to join us at any moment - if you are not local, just stop by the reception desk and ask for the conference; the receptionists will let us know and we will come for you. The end of the conference together with presentation of awards is planned to be around 16:30. For more details, please see the general programme or a time-table with list of presenting authors.

Presentations

The presentations are to be made in english and should last 10 min. Each presentation will be followed by a Q&A.

In person participants will have the opportunity to upload their pptx or pdf files on a computer durting the coffe break prior to their session.
Virtual participants will share their presentation from their computer. In the hybrid form, virtual participants are encorugaed to secure a calm enviroment with good connection as well as to share their video.

Best presentation awards

The chairpersons and the organizers had the pleasure to present the following doctoral candidates with prices for the best presentation in their sections:

  • Organic Chemistry: Killiann Heinz (University of Chemistry and Technology, Prague)
  • Biochemistry: Ondřej Morávek (University of Pardubice)
  • Environmental Chemistry: Jan Čundrle (J. E. Purkyně University in Ústí nad Labem)
  • Analytical, Physical and Computational Chemistry: Jorge Servert Lerdo de Tejada (University of Manchester)
  • Chemical Engineering and Technology: Dániel Gardenö (University of Chemistry and Technology, Prague)

Conference proceedings

The orginisers wish to thank to all participants who submited their abstracts which were collected in the IDCAC 2024 Book of abstracts. The publication with ISBN: 978-80-7592-269-4 is available here. The full sugested citation is: E. Pospíšilová, S. Hermochováa J. Vališ, „The 1st International Doctoral Conference on Advances in Chemistry – book of abstracts", Prague, Czechia,  2024. doi: 10.5281/zenodo.14015303.

Conference partners

The orginisers wish to thank to both UCT Prague, ORLEN Unipetrol Foundation and Zentiva for their generous support.

 ◳ Partners (png) → (originál)

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What is onboarding?

Onboarding is a key process of integrating new employees into the organisation, thereby enhancing both their professional, social, cultural and organisational integration. As a result, employees feel welcomed as part of the organisation and better prepared for the tasks ahead of them. The sooner the new employee feels welcome and fully informed, the better and quicker they will be able to contribute to the organisation successfully.

Onboarding procedures are common in the private sector but often absent in academic institutions. In particular for newly hired Early Career Researchers (ECR), namely PhD candidates, such procedures will strongly impact both their integration into the organisation and clarification of roles and responsibilities of all stakeholders involved in their programme. In order to maximize their impact, it is therefore important that onboarding procedures are approved and supported by supervisors and Postgraduate offices. While it has been shown that successful onboarding contributes to increased well-being and motivation, many ECRs often feel somewhat lost and poorly supported when entering academia, leading to doubt, insecurity and sometimes even an early abandonment of their research project.

UCT and onboarding

In collaboration with the PRIDE network (Professionals in Doctoral Education), a working group was formed with the aim of drafting a publication on onboarding, focusing for the first time on doctoral candidates and postdocs and their adaptation process at the university.

UCT Prague initially launched an extensive questionnaire to assess awareness of onboarding, whether and how many people have encountered it, and what they would welcome within the academic environment. After evaluating the questionnaire, meetings were held in the private sector with HR professionals. We wanted to verify how onboarding is conducted in private companies and how we can leverage their know-how and experiences in our context.

The collected materials and interviews with HR professionals were used to create an adaptation plan, which was piloted from September 2023 at three institutes of  UCT Prague. The resulting adaptation plan, which is part of this page, will serve the needs of UCT Prague supervisors who work with newly hired doctoral candidates. Furthermore, the adaptation plan will be utilized for the PRIDE network publication, serving as a foundation for other European universities.

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Zde se zobrazují boxy (ze složky phd-boxy)

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UCT Prague cooperates with partner universities in Europe on joint doctoral study programmes.

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What is a “Double-Degree” programme?

It is doctoral study programme that is leading to two diplomas from both home university as well as partner university. Student works on one dissertation, the study programmes are fully integrated between collaborating universities and include specialized courses at home university and one interdisciplinary at partner university. 

Each successful applicant signs individual contract about doctoral double degree based on his/her particular situation (research area, supervisor’s requirements, home university requirements etc.). The contract is signed between UCT Prague and student’s home university.

Requirements for doctoral double degree contract:

  • Student has to agree with two supervisors – one from the home university, one from UCT Prague
  • Student has to be accepted by his/her supervisor to PhD studies at UCT Prague
  • Student has to be accepted to PhD studies at his/her home university
  • UCT Prague and student’s home university have to have a joint double degree study programme (list of offered programmes below)

Contact persons at UCT Prague

First contact (study matter) - Contact person of each double degree study programme.

Second contact (general matter, information about the application proceedings)– Department of International Relations: international@vscht.cz

Finances

In terms of cooperation with partner universities no tuition is required. Students are expected to cover any additional costs themselves.

What are the advantages?

Besides improved employability of graduates, there is emphasis on research activity coupled with a possibility to lead small research groups. The program inherently strengthens professional language knowledge. There are possibilities to gain practical skills thanks to cooperation with partner companies such as Zentiva, Zeva, Contipro, Lonza Biotec or in practically oriented departments of Czech Academy of Sciences, where are practical traineeships available. Last but not least there is excellent support of students, including motivational scholarships.

Double degree programmes offered

There are 8 programmes at 4 faculties

FCT – Faculty of Chemical Technology

FET – Faculty of Environmental Technology

FBT – Faculty of Food and Biochemical Technology

FCE – Faculty of Chemical Engineering

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Institute of Microbiology of the CAS

Advanced glycomimetics in neurodegeneration research

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: prof. Ing. Vladimír Křen, DrSc.
Expected Form of Study: Full-time
Expected Method of Funding: Salary

Annotation


Alzheimer's disease (AD) represents a significant health and socioeconomic challenge. This project aims to develop innovative multi-targeted therapeutic approaches through the creation of dual/hybrid compounds. These compounds will combine optimized inhibitors of O-N-acetyl-?-D-glucosaminidase (OGA) and acetylcholinesterase (AChE) inhibitors using covalent conjugation via an optimized linker. Targeting both key biological receptors involved in AD progression offers the potential for more effective therapy. The study will include an evaluation of carbacyclic glycomimetics developed within the Czech-Austrian-Croatian collaboration, including testing their permeability across the blood-brain barrier (BBB) using static and dynamic models, increasing O-GlcNAcylation, and reducing Tau protein hyperphosphorylation. Advanced models of human AD, such as brain organoids, and in vivo efficacy testing in tauopathic mouse models will also be part of the research in collaboration with the Institute of Experimental Medicine of the Czech Academy of Sciences. The project combines cutting-edge research with practical applications and provides a unique opportunity to engage in a multidisciplinary team.

Central Nervous System diagnostics

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: prof. Ing. Vladimir Havlíček, Dr.
Expected Form of Study: Full-time
Expected Method of Funding: Salary

Annotation


Central nervous system infections cause acute changes in mental and physical brain functions, leading to chronic impairment of cognitive, memory, and motor abilities. These changes are related to alterations in brain neuronal signaling in susceptible brain regions. This Ph.D. topic aims to utilize matrix-assisted laser desorption/ionization-mass spectrometry imaging and highly multiplex immunohistochemistry-based mass spectrometry imaging to analyze and quantify molecular interactions between host and pathogen in cases of neuroaspergillosis caused by Aspergillus fumigatus and pneumococcal meningitis caused by Streptococcus pneumoniae. The Ph.D. project will determine the optimal transport mechanism for microbial secondary metabolites, such as toxins and quorum sensing molecules, across the blood-brain barrier. The study will analyze the impact of microbial metabolites on neurotransmitter signaling, specifically through the dopaminergic and glutamatergic pathways, in critically affected brain regions. The candidate biomarkers will successfully diagnose CNS infections in human cerebrospinal fluid samples based on the host-pathogen molecular fingerprint.

Defined chitosan conjugates for biomedical applications

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: doc. RNDr. Pavla Bojarová, Ph.D.
Expected Form of Study: Full-time
Expected Method of Funding: Scholarship

Annotation


Chitin is the second most abundant polymer in nature. Partial deacetylation of chitin produces chitosan, a linear polymer composed mainly of glucosamine units (GlcN) and to a lesser extent of N-acetylglucosamine units (GlcNAc) linked ?(1?4) by glycosidic bonds. Chitosan is biocompatible and can therefore be advantageously used in a variety of biological and biomedical applications. For many biological applications, it is advantageous to work with shorter chains of chitosan, the so-called chitooligosaccharides (COS). COS have been intensively researched for decades in the fields of medicine, pharmaceuticals, textile industry, food industry and agriculture. Despite their tremendous potential use, poorly characterized heterogeneous mixtures are used in most studies due to the unavailability of well-defined COS. The thesis will focus on the preparation of authentic, pure and fully structurally characterized COS and chitosans and their analysis. Subsequently, the defined COS chains will be used as carriers for multivalent presentation of bioactive carbohydrates, and their bioactivities will be studied, especially in biological tests with lectins.

Degradation of bacterial signaling molecules by rational engineered Ntn-hydrolases as novel antibacterials

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biotechnology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: RNDr. Andrea Palyzová, Ph.D.
Expected Form of Study: Full-time
Expected Method of Funding: Scholarship

Annotation


Growing antibiotic resistance will result globally in 10 million deaths annually by 2050. The targeted design of quorum-quenching (QQ) enzymes, negatively affecting the communication process in Gram-negative bacteria leading to biofilm formation, known as quorum sensing (QS) process, represents a clever strategy to effectively combat this threat. Construction and development of modified proteins from the Ntn-hydrolase group (e.g., ecPGA isolated from Escherichia coli) using genetic-molecular techniques (RF cloning, PCR-based methods, CRISPR/Cas technology) and biotechnological strategies (scale-up process, His tag purification) will lead to the develop of a unique QQ enzyme with properties for specific binding to a wider spectrum of HSL molecules (3-oxo-C12-HSL, C8-HSL, C6-HSL, C4-HSL). The designed modified enzymes will be used in in vitro interactions with selected HSL molecules, and their degradation activity will be monitored. Degradation efficiency will be tested in a biological model during biofilm development and maturation in significant HSL-dependent Gram-negative pathogens Pseudomonas aeruginosa, Klebsiella pneumoniae, and Aeromonas veronii. Biofilm morphology will be analyzed using electron microscopy, while the profile of signaling molecules synthesized in the bacterial population will be thoroughly examined using liquid chromatography with tandem mass spectrometry. The project will explore and enhance the biotechnological potential of these QQ enzymes to act as an alternative and complement to conventional antibiotic therapies and agents for medical and industrial applications in combating biofilm formation.

Enzymatic synthesis of biologically active chitooligomers

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: doc. RNDr. Pavla Bojarová, Ph.D.
Expected Form of Study: Full-time
Expected Method of Funding: Scholarship

Annotation


Chitooligomers are ?-1-4-linked oligosaccharides composed of N-acetylglucosamine and glucosamine units and their biological activity depends mainly on their degree of polymerization, degree of acetylation, and acetylation pattern. Chitooligomers are known for their ability to induce an immune response in plants and could thus be used as natural crop protection agents against microbial pests. This PhD project will focus on the efficient enzymatic preparation of chitooligomers with different degrees of polymerization and acetylation, in particular chitooligomers with degrees of polymerization 6-10 and different degrees of acetylation are in demand and not yet available. Microbial natural and mutant glycosidases, particularly chitinases and ?-N-acetylhexosaminidases, and chitin deacetylases from bacterial and fungal sources will be used for this purpose. These enzymes will be expressed in microbial expression systems (E. coli, P. pastoris), characterized, and optimized for the synthesis of the desired chitooligomers. The biological activities of the prepared chitooligomers, especially concerning plant pest protection, will be tested in collaboration with Czech and foreign partners of the laboratory.

Flavonoid-derived metabolites as standards for metabolic studies

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Chemistry of Natural Compounds
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: doc. Ing. Kateřina Valentová, Ph.D.
Expected Form of Study: Full-time
Expected Method of Funding: Scholarship

Annotation


Flavonoids are plant food components that are known to be intensively metabolized in the gut and to interact with and influence the composition of the gut microbiome. Flavonoids are also subject to rapid biotransformation in the II phase, whereby mainly corresponding sulfates and glucuronides are formed. The biotransformation of polyphenols by the gut microbiota also leads to cleavage of the C-ring and the formation of simple phenolic substances. The aim of this PhD project is the optimization of chemoenzymatic methods for the synthesis of a library of natural flavonoids (myricetin, luteolin, kaempferol, naringenin, hydroxyphenylacetic acid and hydroxyphenylpropionic acid) and their metabolites (sulfates, glucuronides and depsides). Natural and mutant enzymes from bacterial and fungal sources (arylsulfotransferases, glucuronidases, quercetinase) are used for this purpose. These enzymes are expressed in microbial expression systems (E. coli, P. pastoris), characterized and optimized for the synthesis of the desired metabolites. The compounds are then used as standards for metabolic studies carried out in collaboration with our partner laboratories.

Changes in the urinary microbiome and metabolome in response to the therapeutic intervention in female patients with overactive bladder symptoms

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biotechnology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: RNDr. Andrea Palyzová, Ph.D.
Expected Form of Study: Full-time
Expected Method of Funding: Scholarship

Annotation


Research into the microbiome of the urinary tract has progressed dramatically over the last decade. Currently, more than 500 bacterial species have been identified in urine, indicating significant diversity in the microbiome of the niche. As our understanding of the urinary microbiome grows, there is evidence of differences in microbiome composition between healthy individuals and those with lower urinary tract diseases. Overactive bladder (OAB) is a chronic disease of the lower urinary tract dysfunction affecting up to 20% of the population, more often women than men, characterized by sudden urges to urinate with a negative impact on quality of life. The pathophysiology of OAB is multifactorial, including metabolic syndrome, affective disorders, sex hormone deficiency, and other factors. The standard treatment of OAB is mainly based on lifestyle modification, behavioural therapy, and pharmacotherapy with anticholinergics. This study aims to correlate characteristics of the urinary microbiome and metabolome with OAB symptom severity and then to evaluate changes in the urinary microbiome and metabolome in response to therapeutic intervention for OAB. The study will include 210 subjects, approximately 60 with OAB symptoms and 150 healthy controls. Inclusion criteria for the OAB group will be the presence of clinical symptoms, the OAB-V8 patient-reported outcome questionnaire and the nerve growth factor (NGF) levels. Standard molecular techniques of DNA isolation and purification, PCR amplification, and sequencing by massively parallel sequencing on the Illumina MiSeq platform, complemented by bioinformatic analysis, will be used to gain insights into the microbiome. In addition to the composition of the microbial community, the molecular mechanisms at the level of identification of specific low molecular weight metabolites will play a key role in the patient´s condition. We will use non-targeted metabolomics to obtain comprehensive molecular profiles and semi-quantitatively determine the levels of unique metabolites. This will provide new insights into the biochemical functions of the detected metabolites associated with OAB and identify potential biomarkers associated with the development of this disease.

Integrated Stress Response in survival or cell death.

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: Mgr. Leoš Valášek, Ph.D.
Expected Form of Study: Full-time
Expected Method of Funding: Scholarship

Annotation


Eukaryotické buňky si vyvinuly několik mechanismů, jak se vyrovnat s různými stresory prostředí. Patří mezi ně komplexní signální dráha označovaná jako integrovaná stresová odpověď (ISR), která vede buňky buď k adaptaci nebo ke smrti. Zatímco jejími vnějšími spouštěči jsou kyslík, nedostatek živin nebo virové infekce, hlavním vnitřním stresorem je hromadění nesložených proteinů v lumen endoplazmatického retikula (ER). ISR může být také indukována aktivací onkogenů. Reakce je „integrovaná“, protože všechny stresové signály jsou přenášeny rodinou čtyř serinových/treoninových kináz a konvergují do jediné události, kterou je fosforylace ? podjednotky eukaryotického iniciačního faktoru translace 2 (eIF2?). Provedli jsme komplexní screening, abychom zjistili, jaké mRNA a lncRNA jsou překládány za normálních podmínek versus akutní nebo chronické (pro-apoptotické) ISR. Cílem navrhovaného projektu je vybrat klíčové mRNA z našeho robustního screenu a prozkoumat jednak molekulární mechanismus jejich specializované translace, jakož i úlohu jejich proteinových produktů za daných stresových podmínek.

Secondary metabolism of intracellular pathogens

Study place: Institute of Microbiology of the CAS
Guaranteeing Departments: Department of Biochemistry and Microbiology
Institute of Microbiology of the CAS
Study Programme/Specialization: ( in English language )
Supervisor: prof. Ing. Vladimir Havlíček, Dr.
Expected Form of Study: Full-time
Expected Method of Funding: Salary

Annotation


The PhD candidate will study, correlate and mine the metabolism of intracellular pathogens within host cells. The candidate can solve five pressing diagnostic problems of our time, and together with the Stanford University, can address the dynamic range of analytical chemistry in mapping and quantifying intracellular pathogens in the host. Through this collaboration, the applicant will be able to work with isogenic strains, i.e., will always have host systems with very similar metabolomics and identical host genetic makeup. All directions of intracellular research are of the highest societal importance and involve the endemic fungus Coccidioides immitis, a Pf4 bacteriophage in the Gram-negative bacterium Pseudomonas aeruginosa, the AfPmV-1 polyomavirus in the filamentous fungus Aspergillus fumigatus, and the betaproteobacterium Mycetohabitans endofungorum, an intracellular symbiont of the filamentous fungus Rhizopus microsporus. Also a contribution to a novel diagnostic for Mycobacterium tubercullosis in mammalian cells is expected. The applicant will use the portfolio of infection metallomics, a set of dependent mass spectrometry, separation, and computational techniques. ) ) [sablona] => stdClass Object ( [class] => stranka [html] => [css] => [js] => [autonomni] => 1 ) [api_suffix] => druh/I/jazyk/en/locale/en/ustav/004 )

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